Esports is characterised by its repetitive movements, and excessive engagement in this activity may lead to the development of gaming disorders and contribute to a range of musculoskeletal conditions. This study aimed to evaluate the ergonomic risk factors associated with musculoskeletal disorders using the RULA and ROSA methods among professional Esports players. A cross-sectional study was carried out among Malaysian Esports players in Kuala Lumpur (n=84) from September 2021 to June 2023. Data was collected using a self-administered questionnaire. The posture of the participants was assessed using the RULA method, strain related to computer-based hazards and workstation environment via the ROSA method, participants’ internet gaming disorder was recorded using the Internet Gaming Disorder Scale (IGDFS9-SF), and the range of the musculoskeletal pains was assessed using the Nordic Musculoskeletal Questionnaire (NMQ). Data analysis was done using descriptive statistics and logistic regression. A total of 15 (17.86%) Esports players were reported to having IGD. Based on the NMQ, the common musculoskeletal pain due to gaming during the past 12 months was related to the hand/wrist, 33 (39.2%), neck, 27 (32.1%), and shoulder, 22 (26.2%). Among the players with IGD, 9 (60%) complained of having MSD. Ergonomic assessment via RULA showed 44 (52.4%) players were classified as having high ergonomic risk, with 25 (56.8%) players were significantly associated with MSD (p = 0.045; p<0.05). ROSA method indicated that 50 (59.5%) players were classified as having a high ergonomic risk with 21 (42%) players were found to have MSD, however, the association was not statistically significant (p = 0.324, p>0.05). Esports players are at a greater risk of getting musculoskeletal complications as a result of adopting improper posture and being exposed to a hazardous working environment.  There is a significant need for interventions and increased knowledge of ergonomics to encourage better gaming habits.